| Professional
Experience |
| University of Iowa, College of Pharmacy, Assistant
Professor, 1969-1972 |
| University of Iowa, College of Pharmacy, Associate
Professor, 1972-1977 |
| University of Iowa, College of Pharmacy, Professor,
1977- |
| Research |
|
Dr. Rosazza's
research interests are focused on the fields of biocatalysis,
natural products biochemistry and chemistry, and microbiology.
He has investigated the microbial, enzymatic, and chemical transformations
of antitumor alkaloids, antibiotics, and steroids, and herbicides
and pesticides. He has developed microorganisms for use as models
of mammalian, plant, and insect metabolism and for applications
as reagents in organic chemistry. Currently, Rosazza is researching
the enzymology, biochemistry, and chemical mechanisms of nitric
oxide formation in bacteria; the identification of abortifacient
principle(s) of Pinus ponderosa; biocatalytic mechanisms of
metabolism of antioxidants including tocopherols and ferulic
acid; the development of decarboxylases and carboxylic acid
reductases as biocatalysts for organic syntheses; and uses of
microbial models to predict metabolism of xenobiotics in the
environment. |
| Representative
Publications |
| Janelle Torres y Torres and John P. N. Rosazza.
2001. Reactions of p-coumaric acid with nitrite: product isolation
and mechanism studies. J Ag Food Chem 49: 1486-1492. |
| Mohammed Hosny, Kajari Dhar and John P. N. Rosazza.
2001. Microbial transformation of quercetin, fisetin and catechin
by Streptomyces griseus. J Nat Prod 64: 462-465 |
| Janelle Torres y Torres and John P.N. Rosazza. 2001
Microbial transformations of p-coumaric acid by Bacillus megaterium
and Curvularia lunata.. J Nat Prod 64:1408-1414. |
| S. Gough, M. Deshpande, M. Scher and JNP. Rosazza.
2001. Permeabilization of Pichia pastoris for Glycolate Oxidase
Activity. Biotechnology Letters 23: 1535-1537. |
| Alok Dhar, Kahari Dhar and John P. N. Rosazza. 2002.
Purification and properties of an enoyl-CoA hydratase from Galactomyces
reessii. J Indust Microbiol Biotech 28: 81-87 |
| Kyeung Seon Lee and John P. N. Rosazza. 2002. Biocatalytic
oxidations of 4-vinylphenol by Nocardia spp. Can J Chem 80: 582
– 588. |
| Mohammed Hosny and John P. N. Rosazza. 2002. Isolation
and characterization of novel flavonoid and triterpene glycosides
from Novasoy. J. Nat Prod 65: 805 – 813. |
| Mohammed Hosny, Holly A. Johnson, Amanda K. Ueltschy
and John P. N. Rosazza. 2002. Oxidation, reduction and methylation
of carnosic acid by Nocardia. J. Nat Prod 65: 1266-1269. |
| Kajar Dhar, Alok Dhar and John P.N. Rosazza. 2003.
Glutathione-S-transferase isoenzymes from Streptomyces griseus.
Appl Environ. Microbiol 69: 707-710 |
| John P. N. Rosazza and Mohammed Hosny. 2002. Isoflavone
and triterpene glycosides from soybeans. US Patent Application
10/126,483 – Patent Pending - Filed April 19, 2002 |
| John P. N. Rosazza and Yijun Chen. 2003. Purified
Nitric Oxide synthase. United States Patent # 09/225,426, filed
January 5, 1999 – pending. |
| Aimin He and John P. N. Rosazza. 2003. Microbial
transformations of naproxen by Aspergillus niger ATCC 9142. Pharmazie
58:420-422 |
| John P. N. Rosazza, Lacy Daniels, Aimin He, Tao
Li and Ian Fotheringham. 2003. Cloning and Expression of Nocardia
sp. NRRL 5646 GTP Carboxylic Acid Reductase (Aldehyde Oxidoreductase).
Patent Application Pending |
| Monica Sanchez-Gonzalez and John P. N. Rosazza.
2003. Mixed aromatic acyloin condensations with recombinant benzaldehyde
lyase: Synthesis of ‚-hydroxydihydrochalcones and related
alpha-hydroxyketones. Adv. Syn. Catal. 345:819-824. |
| Aimin He and John P. N. Rosazza. 2003GTP Cyclohydrolase
I: Purification, characterization and effects of inhibition on
nitric oxide synthase in Nocardia sp. Appl. Environ. Microbiol.
69(12):7507-7513, 2003 |
| Aimin He, D. Randall Simpson, Lacy Daniels and John
P.N. Rosazza. 2003. Cloning, expression, purification and characterization
of Nocardia sp. GTP cyclohydrolase I. Protein Expression and Purification
(accepted) |
| John P.N. Rosazza, Aimin He, Tao Li, Ian Fotheringham,
Lacy Daniels. 2002. Cloning and expression of carboxylic acid
reductase (CAR), a biocatalyst for reductions of carboxylic acids
to aldehydes. Disclosure filed June, 2002. (UI Research Foundation) |
| John P.N. Rosazza, Aimin He, Tao Li, Ian Fotheringham,
Lacy Daniels. 2003. Carboxylic acid reductase polypeptide, nucleotide
sequence encoding same and methods of use. US. Patent Serial No.
10/386,329 filed March 11, 2003 |
| Monica Sanchez-Gonzalez and John P. N. Rosazza.
2003. O-Demethylation, hydroxylations and cyclizations of chalcones
by Asergillus alliaceus. J.Nat. Prod. (In Press) |
| John P.N. Rosazza, Mohammed Hosny and Holly Johnson.
2002. Oxidation, Reduction, and Methylation of Carnosic Acid by
Nocardia Yielding Two Novel Chemical Structures. Invention disclosure
and patent prepared. ( With Kemin Industries) |
| Aimin He, Lacy Daniels, Tao Li, Ian Fotheringham
and John P. N. Rosazza. 2003. Cloning and expression of Nocardia
sp. carbozylic acid reductase(aldehyde exidoreductase). Appl.
Environ. Microbiol. (In Press) |
| Sungwon Lee and John P. N. Rosazza. 2003. Total
synthesis of Mycothiol: The major low molecular weight thiol in
Nocardia species. Org. Letters (In Press) |
| Grant |
| National Science Foundation, Engineering Research
Center - Center for Environmentally Beneficial Catalysis. J. Rosazza
is Director of the University of Iowa component focusing on Oxidative
Biocatalysis. Total $3.57 million - 9/1/03 - 8/31/08 |
