Medicinal and Natural Products Chemistry

Kevin G. Rice Division of Medicinal and Natural Products Chemistry College of Pharmacy, University of Iowa Iowa City, Iowa tel: 319-335-9903 fax: 319-335-8766 email: kevin-rice@uiowa.edu Rice Research Presentation
Education
B.S., Chemistry & Biology, Marycrest College, Davenport, Iowa, 1983 Ph.D., Medicinal & Natural Products Chemistry, College of Pharmacy, The University of Iowa, 1987

Professional Experience

Postdoctoral Fellow, Department of Biology, The Johns Hopkins University, Baltimore, Maryland, 1987-91

Assistant Professor of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio, 1991-94

Assistant Professor of Medicinal Chemistry and Pharmaceutics, College of Pharmacy, The University of Michigan, Ann Arbor, Michigan, 1995-97

Associate Professor of Medicinal Chemistry and Pharmaceutics, College of Pharmacy, The University of Michigan, Ann Arbor, Michigan, 1997-2001

Professor and Division Head, Medicinal & Natural Products Chemistry, Professor of Pharmaceutics, College of Pharmacy, The University of Iowa, Iowa City, Iowa, 2001-

Awards

American Chemical Society Undergraduate Summer Research Fellow, Wichita State University, 1983

Dimitri V. d'Arbeloff Postdoctoral Fellow, Department of Biology, The Johns Hopkins University, 1987

National Research Service Award, Sponsored by the NIH-GMS, Department of Biology, The Johns Hopkins University, 1988

Horace S. Isbell Award, American Chemical Society, Division of Carbohydrates, 2001

Inducted into The Johns Hopkins Society of Scholars, 2003

Research

Dr. Rice's research interests are in the synthesis and testing of carrier systems for targeted gene delivery. His studies involve peptide, carbohydrate and bioconjugate synthesis, gene formulation, in vitro and vivo testing of gene delivery systems.

Representative Publications

Martin, M.E. and Rice, K.G. “A Novel Class of Intrinsic Proteasome Inhibitory Gene Transfer Peptides.” Bioconj Chem. (2008) 19(1):370-376.

Rettig, G.R. and Rice, K.G. "Non-viral gene delivery: from the needle to the nucleus.” Expert Opin Biol Ther. (2007) 7(6):799-808.

Chen, C.P., Kim, J.S., Liu, D., Rettig, G.R., McAnuff, M.A., Martin, M.E., and Rice, K.G., “Synthetic PEGylated Glycoproteins and their Utility in Gene Delivery.” Bioconj. Chem. (2007) 18(2):371-8.

McAnuff, M.A., Rettig, G.R., and Rice, K.G. “Potency of siRNA versus shRNA Mediated Knockdown in Vivo”, J. Pharm. Sci. (2007) 96(11):2922-2930.

Martin, M.E. and Rice, K.G. “Peptide Guided Gene Delivery.” AAPS J. (2007) 9(1):E18-29.

Rettig, G.R., McAnuff, M., Liu, D., Kim, J.S., and Rice, K.G., “Quantitative Bioluminescence Imaging of Transgene Expression In Vivo.” Anal. Biochem. (2006) 335(1):90-94.

Chen, C.P., Kim, J.S., Steenblock, E., Liu, D., and Rice, K.G., “Gene Transfer with Poly-melittin Peptides.” Bioconj. Chem., (2006) 17(4):1057-1062.

Oyewumi, M.O. and Rice, K.G. “DNA Nanoparticle Gene Delivery Systems” in Nanotechnology for Drug Delivery, Marcel Dekker, Inc., Eds, R.B. Gupta and U.B. Kompella. (2006) Ch. 12, pp. 361-379.

Huang, Y.C., Riddle, K., Rice K.G., and Mooney, D.J. “Long-term In Vivo Gene Expression via Delivery of PEI-DNA Condensates from Porous Polymer Scaffolds.” Hum Gene Ther (2005) 16(5):609-617.

Huang, Y.C., Simmons, C., Kaigler, D., Rice, K.G., and Mooney, D.J. “Bone Regeneration in a Rat Cranial Defect with Delivery of PEI-Condensed Plasmid DNA Encoding for Bone Morphogenetic Protein-4 (BMP-4).” Gene Ther (2005) 12(5):418-426.

Kim, J., Chen, C.P. and Rice, K.G. “The Proteasome Metabolizes Peptide-Mediated Nonviral Gene Delivery Systems.” Gene Ther., (2005) 12(21):1581-90.

Huang, Y.C., Kaigler, D., Rice, K.G., Krebsbach P.H., and Mooney, D.J. “Combined angiogenic and osteogenic factor delivery enhances bone marrow stromal cell-driven bone regeneration.” J. Bone and Miner Res (2005) 20(5):848-57.

Elhalabi, J. and Rice, K.G. “Synthesis of Uridine 5’-[2-S-pyridyl-3-thio-a-D-galactopyranosyl diphosphate]: Precursor of UDP-Thiogal Sugar Nucleotide Donor Substrate for b-1,4-Galactosyltransferase.” Nucleosides, Nucleotides & Nucleic Acids (2004) 23(1-2):195-205.

Thanawiroon, C., Rice, K.G., Toida, T., and Linhardt, R.J. “Liquid Chromatography/Mass Spectrometry Sequencing Approach for Highly Sulfated Heparin-derived Oligosaccharides.” J. Biol. Chem. (2004) 279(4):2608-2615.

Chen, C.P., Park, Y. and Rice, K.G. “An Improved Large-Scale Synthesis of PEG-Peptides for Gene Delivery.” J. Pept Res (2004) 64(6):237-243.

Rice, K.G., Kim, J.S., and Liu, D. “Glycopolymers: Tools for Studying Targeted Non-viral Gene Delivery” in Polymeric Gene Delivery, (Mansoor M. Amiji Ed.) CRC Press (2004) pp. 509-521.

Kwok, K.Y., Park, Y., Yang, Y., McKenzie, D.L., Liu, Y. and Rice, K.G. “In Vivo Gene Transfer using Sulfhydryl Cross-linked PEG-peptide/Glycopeptide DNA Co- Condensates.” J. Pharm. Sci. (2003) 92(6):1174-1185.

Huang, Y.C., Connell, M., Park, Y., Mooney, D.J. and Rice, K.G. “Fabrication and In Vitro Testing of a Polymeric Delivery System for Condensed DNA.” J. Biomed. Mater. Res. (2003) 67(4):1384-1392.

Rice, K.G., Thomas, V.H. and Yang, Y. “Probing the Binding Specificity of C-Type Lectins In Vivo.” Methods Enzymol. (2003) 363:90-104.

Park, Y., Kwok, K.Y., Boukarim, C. and Rice, K.G. “Synthesis of Sulfhydryl Cross-linking Poly(ethylene glycol)-Peptides and Glycopeptides as Carriers for Gene Delivery.” Bioconj. Chem. (2002) 13(2):232-239.

Elhalabi, J. and Rice, K.G. “Thiosugar Nucleotide Analogs: Synthesis of 5’-(2,3,4-tri-O-acetyl-6-S-acetyl-6-thio-alpha-D-galactophyranosyl diphosphate).” Carbohydr. Res. (2002) 337(21-23):1935-1940.

Rice, K.G. "Preparative Purification and Utilization of Tyrosinamide N-Glycan Libraries" in Carbohydrate Analysis by Modern Chromatography and Electrophoresis, Journal of Chromatography Library, Elsevier, Amsterdam, The Netherlands, (2002) Vol 66, pp 535-570.

Kwok, K.Y., Yang, Y.S., and Rice, K.G. “Evolution of Cross-linked Non-viral Gene Delivery Systems” Curr Opin Mol Ther (2001) 3(2):142-146.

Yang, Y., Park, Y., Man, S., Liu, Y. and Rice, K.G. "Cross-linked Low Molecular Weight Glycopeptide-Mediated Gene Delivery: Relationship between DNA Metabolic Stability and the Level of Transient Gene Expression In Vivo" J. Pharm. Sci. (2001) 90(12):2010-2022.

Elhalabi, J. and Rice, K.G. “Thiosugar nucleotide analogues: synthesis of uridine 5`-(2,3,6-tri-O-acetyl-4-S-acetyl-4-thio-alpha-D-galactopyranosyl diphosphate)” Carbohydr. Res. (2001) 335(3):159-165.

Rice, K.G. “Application of Fluorescence Resonance Energy Transfer to Analyze Carbohydrates.” Anal. Biochem. (2001) 297(2):117-122.

Collard, W.T., Evers, D. L. McKenzie D.L. and Rice, K. G. “Synthesis of Homogenous Glycopeptides as DNA Targeting Agents.” Carbohydr. Res. (2000) 323(1-4):176-184.

Collard, W.T., Yang, Y., Kwok, K.Y., Park, Y. and Rice, K.G. “Biodistribution, Metabolism, and In Vivo Gene Expression of Low Molecular Weight Glycopeptide Polyethylene glycol DNA Co-Condensates.” J. Pharm. Sci. (2000) 89(4): 499-511.

McKenzie, D.L., Kwok, K.Y. and Rice, K.G. “A Potent New Class of Reductively Activated Peptide Gene Delivery Agents.” J. Biol. Chem. (2000) 275(14):9970-9977.

Rice, K.G. “Derivatization Strategies for Preparing N-Glycan Probes.” Anal. Biochem. (2000) 283(1):10-16.

Kwok, K.Y., Adami, R.C., Hester, K.C., Park, Y., Thomas, S., and Rice, K.G. “Strategies for Maintaining the Particle Size of Peptide DNA Condensates Following Freeze-drying.” Int. J. Pharm. (2000) 203(1-2):81-88.

McKenzie, D.L., Smiley, E., Kwok, K.Y., and Rice, K.G. “Low Molecular Weight Disulfide Cross-linking Peptides as Nonviral Gene Delivery Carriers.” Bioconj. Chem. (2000) 11(6):901-909.

Yang, Y.S., Thomas, V.H., and Rice, K.G. “Tissue Targeting of Multivalent GalNAc-Lex Terminated N-glycans in Mice.” Glycobiology (2000) 10(12):1341-1345.

College of Pharmacy
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